Due to very high humidity inside the culture vessel and artificial conditions of development, the
plantlets are tender and are therefore are not ready for coping up with the filed conditions. The
plants removed from the sterile medium are washed and are maintained under intermittent mist
or are covered with clean transparent plastic. After 10 to 15 days under high humidity, the
plants are transferred to green house and maintained for another 4 to 6 weeks. They are then
ready to be transferred to net house or the field. Normally, the tissue culture plants are sold
either as ex-agar plants or hardened plants from the green house.
Ex-agar plants
Depending on the parameters such as location/the site of planting, soil quality
and the climatic conditions defined by the customer, the ex-agar plant for sale
could be in vitro rooted plants or only the shoots. When the tissue culture plants
are sold at this stage, the plants are washed in sterilized water to remove the
agar medium. The plants after being removed from nutrient media should preferably be
transplanted within 72 hours.
Shadenet/Green House Room
The plants are transferred to net pots! pro tray for acclimatization after they fully develop shoots
and roots in the bottles. The rooted plantlets are transferred to pots filled with suitable substrate
and are watered. This operation is carried out on an open bench. These pots are then
transferred to the green house for 4 to 6 weeks. During this process, they are given fertilizers
and treated like plantlets obtained by any other means of propagation. After the plants are
acclimatized fully, they are transferred to poly-bags. At this stage the plants are completely
hardened and are ready to be planted in the field for cultivation. Hardening units can be set up
in sites away from the micro propagation unit.
We have 20,000 Sq. Mts of Shade House area for the secondary hardening as well rowing of
various kinds of plants under insect free conditions.
